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Indirect immunofluorescence using salt-split skin:
| • | induces split at the level of the lamina lucida |
| • | autoantibodies in patients serum react to either the roof (epidermal side) or the floor (dermal side) of the split to help with diagnsosis: |
| • | EBA - floor (collagen VII) |
| • | bullous SLE - floor (collagen VII) |
| • | cicatricial pemphigoid - roof, floor, or both: roof (BP2 antigen) = most common; floor (collagen VII) = least common |
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